Conference Proceeding

OMICS Insights into Common Bean (Phaseolus vulgaris L.) under Mineral Stress

Dr. Sajad Majeed Zargar

Common bean (Phaseolus vulgaris L.) is a valuable legume food crop nutritionally as well as medicinally. Being a good quality and cheap source of protein, fiber, carbohydrates, minerals and vitamins it has the potential to play a leading role in addressing the issues of malnutrition and hunger-related problems for the poor of the world. As a consequence, it is rightly considered as the “poor man’s meat”. Among various minerals that common bean possesses, iron (Fe) and zinc (Zn) are present in relatively high amount and are important for both plants and animals for a range of critical enzymatic and metabolic reactions. Fe is required for basic redox reactions in both photosynthesis and respiration, and for many key enzymatic reactions involved in important biological processes such as DNA replication, lipid metabolism and nitrogen fixation in plants. Zn is also necessary for various metabolic and physiological processes in plants acting as a cofactor for more than 300 enzymes. Common bean is considered as a legume model to unravel the responses and adaptation to Fe, Zn and P deficiencies1. In our study, we have used GWAS (Genome Wide Association) approach to identify QTLs contributing for accumulation of Fe and Zn in common bean seeds. Further, we tried to examine the impact of Fe deficiency and excess Zn on the leaf proteome of 15-days-old common bean seedlings (variety VLR-125) using gel-based proteomics (2-DE) in conjunction with MALDI-TOF/ MS analysis. Physiological and morphological parameters revealed that Fe deficiency and excess Zn had similar impact on chlorophyll content of shoots, shoot and root length and weight compared to the control condition. For proteomics analysis, selected proteins that were differentially expressed under Fe deficiency and excess Zn compared to control (basal MGRL medium) were studied in detail. A total of 46 proteins were found up-regulated and 26 proteins down-regulated in Fe deficiency compared to control. Similarly, nine proteins were observed up-regulated and six proteins were down-regulated in excess Zn condition compared to the control. Further, seven proteins were observed up-regulated and five proteins down-regulated under both Fe deficiency and excess Zn conditions, indicating the possibility of cross talk under such conditions. Based on cellular compartmentalization, most of the identified proteins were found to be localized to the chloroplast and nucleus, whereas only few identified proteins where found localized in mitochondria, endoplasmic reticulum and plasma membrane. These proteins were found to be involved in various biological functions like carbohydrate metabolism, photosynthesis, defense and stress response, transport, cellular processes (replication, transcription and translation) and cellular respiration. This work provides a comprehensive model to understand the adaptive mechanism used by common bean shoots under Fe deficiency/excess Zn conditions at the protein level. However, further, in-depth proteome analysis is required to understand the cross talk under Fe deficiency and excess Zn in common bean.

Published: 08 November 2017

Copyright:

Copyright: © 2017 Dr. Sajad Majeed Zargar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.