Conference Proceeding

Nano/micro porous bilayer dressings for use as a hemostatic agent

Ms. Zeynep Karahaliloglu,
Aksaray University, Turkey

Uncontrolled hemorrhage is the most important cause of death in battlefield, emergency and hospital settings.

Zeynep Karahaliloglu received a BSc Degree in Biology Education from Hacettepe University, Ankara in 2007. She earned her M.S degree in the Department of Nanotechnology and Nanomedicine at Hacettepe University. Zeynep Karahaliloglu currently working in the Department of Biology at Aksaray University as a research assistant. Her research interests are in the area of biocompatibility of biomaterials, tissue engineering applications and drug delivery systems for cancer therapy.

Uncontrolled hemorrhage is the most important cause of death in battlefield, emergency and hospital settings. %50 of deaths are derived from exsanguination in military settings and %80 of these deaths are the result of noncompressible injuries. Hence, hemorrhage control is imperative to decrease fatality rates and improvements to treatment of hemorrhage is believed to have the greatest potential impact on decreasing postoperative morbidity. Few major clinical methods have been used to achieve hemostasis such as chemical (thromboxane) thermal (electrodes, lasers or heat) and mechanical (pressure or ligature). However, these conventional methods have some limitations, they may induce allergic reactions and unknown infectious diseases as well as having opaque properties as observed in powder-type topical hemostatic agents. The most recent comprehensive reviews related to topical hemostatic agents showed the need for future research and development of improved hemostatic agents and devices. Here, we investigated the hemostatic efficacy of a nano/micro bilayer hemostatic dressing including a porous sublayer from chitosan and bacterial cellulose and a nanofibrillar upper layer from silk fibroin. In relation to in-vivo results, SF coated PS (protamine sulfate)/BC/CTS significantly reduced bleeding time (80±0.3 s) compared to standard gauze and kaolin-doped group (p<0.005). Blood loss and mortality rate with 2.3 g and 37.5%, SF coated BC/CTS and SF coated PS/BC/CTS showed increased efficacy in achieving hemostasis compared to standard gauze. All these data suggest that active agent doped BC/CTS/SF based bilayer hemostatic dressings have a great influence on the resulting hemostatic action and can become unique tool for surgeons with ease of handling and low cost as a hemostatic agent.

Published: 27 April 2017