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Targeted Editing of Avian Herpesvirus Vaccine Vector using CRISPR/Cas9 Nuclease

Research Article

Authors:

Herpesvirus of turkey (HVT) is an avian alphaherpesvirus used as live vaccine against Marek's disease for more than 40 years. Here, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of introducing targeted mutations to the genome of a live herpesvirus vaccine vector using a transfection-infection system.


Published: 15 Nov 2016

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